1955_abramson_4000_1.pdf

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*_imLgD No.If5
.o.1
(§91Q)
.o.5 (§lo2d)
(§9On)
(§102a)
M 500 effect on snails (Ambularia cuprima)
serotonin
ABRA_ON,
H.A.,
JARVIK,
M.E. (Biol.Lab., Cold Spring Harbor)
Lyoer_ic
acid diethylamide (LSD-25)| IX. Effect on _nails.
J.Peychol.40,537(1_55).
We report here or, another reaction which may
possibly provide
a
bio-
assay technique
for LSD-2f
We found that several species of snails respond
t_ LSD-25 but
our
data are mainly concerned with Ambutaria
cuprina_
"fhis species is known in pet shops
as
"Mystery Snail"
and
is
readily obtain_
_bie. Shortly
after
immersion in solutions
of
distilled water
containing
1yLSD-25/_,
the snail
opens
its opereulum, extrudes its tentacles,
proboscis,
a_ld gastropod,
the motion
oi
the
gastropod
being
abnormal.
It consists
of a
rather wild, undulating, waving muscular
movement
which prevents the
snail from
adhering
to
any
surface. Normal response to gentle tactile stimu-
lation is lost but the snail will
dose
to
a
more vigorous stimulation
only
r_ reopen
again
within
a
brief period (Figure 1). This disorganized move-
_r_ent has
been observed
to last for
36
hours
and more
when
snarls usually
d_
This prolonged
action is
similar to
the
prolonged response by
Betta
s/,lendens
and
persists
after
the snails
are removed
to fresh water
or
to
open
a_o Ho_'ever, quite
a number of
the
snails
die rapidly in higher concen-
t,a_ior:
Afte_ two hours immersion in solutions _ntaining
0.1_,/cc
of
LSD_25,
the
same
wave-like phenomena
occult.
After immersion for four
hours in solutions containing
0.01y
LSD-25/ec the snails may then remain
orsen and
show the typical reaction. The fantastic, enveloping, persistent,
wavelike motion of _be gastropod is very different
from
the distorted, stu-
porous state of
the
Betta splendens
although
Bettm,
as
m,ntioned, show a
I_e!imina_,
short
e×citation phase. It should be mentioned that
only
maih
that climb
out
of
an
underwater trap
or
vessel
are
employed to test the
d:ug_ (Figure
2).
Concentrations of BOL-148
above
l_,/ec usually
cause
shutting of the
sr_aiF_ operculum and immobility. Since BOL-148 di_ers
only
by
a
bromine
_:,tr_ on the indole ring this marked difference in response is rather
un-
e_o:edo
In
solutions containing 0.5_¢/cc
atypical
behavior is observed but
n,_rmz_,behavior is shown in
solutions
containing 0.053,cc. In rare instances
a snzil
_eacted
to
BO[,-148 in high concentrations
with
_ reaction
similar
tc LSD-25o Snails tl_at had
been closed
by their respon_ to low doses
of
BOL-148 reacted the next day to LSD-25 typically after immersion in dis-
ti!led water. However, snai1_: resportd
to
LAE-32 similarly to
LSD-25o
Semtonin stepped vigorous
undula6cm of the LSD-25
react/on w/th closure
o_
operculum,
but a/ter a _
a,_ute_ the
mails opened
again and resumed
t.,-_I'A:
•
wog
their
LSD-25
activity
with possibly
s_me
minor
alterations
which coul d
.n_.
be described without
slow motion
pictures°
t
It is
believed
that different mechanisms of
response
may be
involved
in
the
•
°
.
'_
snail
and
in
the fish.
What
these m_ehanisms
are is under
mvesugatam.
w7 (E
8015)
NET/bh 1155
,
,A_SA

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